Molecular Mechanism of Type I Collagen Homotrimer Resistance to Mammalian Collagenases*

نویسندگان

  • Sejin Han
  • Elena Makareeva
  • Natalia V. Kuznetsova
  • Angela M. DeRidder
  • Mary Beth Sutter
  • Wolfgang Losert
  • Charlotte L. Phillips
  • Robert Visse
  • Hideaki Nagase
  • Sergey Leikin
چکیده

Type I collagen cleavage is crucial for tissue remodeling, but its homotrimeric isoform is resistant to all collagenases. The homotrimers occur in fetal tissues, fibrosis, and cancer, where their collagenase resistance may play an important physiological role. To understand the mechanism of this resistance, we studied interactions of alpha1(I)(3) homotrimers and normal alpha1(I)(2)alpha2(I) heterotrimers with fibroblast collagenase (MMP-1). Similar MMP-1 binding to the two isoforms and similar cleavage efficiency of unwound alpha1(I) and alpha2(I) chains suggested increased stability and less efficient unwinding of the homotrimer triple helix at the collagenase cleavage site. The unwinding, necessary for placing individual chains inside the catalytic cleft of the enzyme, was the rate-limiting cleavage step for both collagen isoforms. Comparative analysis of the homo- and heterotrimer cleavage kinetics revealed that MMP-1 binding promotes stochastic helix unwinding, resolving the controversy between different models of collagenase action.

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عنوان ژورنال:

دوره 285  شماره 

صفحات  -

تاریخ انتشار 2010